The Toptica FemtoFiber ultra 920 at an output wavelength of 920 nm is ideally suited for the two-photon excitation of common fluorophores like GFP, eGFP, Eosin, GCaMP, CFP, Calcein or Venus.
In 2-photon microscopy, peak-power is brightness! If you care for the best image brightness, you need short pulses, high power, and most importantly a clean temporal pulse shape.
With more than sufficient output power, shortest pulses, and our unique Clean-Pulse Technology, the FemtoFiber ultra 920 features the highest relative peak power and enables unmatched brightness in 2-photon microscopy without unwanted heating of the sample.
With its robust and compact design, the FemtoFiber ultra 920 is an easy to operate and maintenance-free laser system. The laser system is a great solution for applications in non-linear microscopy like two-photon excitation of fluorescent proteins and SHG based contrast mechanisms. With the emission wavelength of 920 nm it provides highest peak power for especially green and yellow fluorescent protein markers (GFP, YFP) commonly used e.g. in neurosciences and other laser related biophotonics disciplines.
Turn-key and intuitive operation, fully-integrated dispersion compensation to ensure shortest pulses at the sample and built-in power control make the system extremely user-friendly and allow you to focus on your research!
The unique offering of the FemtoFiber ultra 920 has the potential to revolutionize two-photon fluorescence microscopy by making simple and cost-efficient light sources available to everyone.
Watch webinar: Setting Up a Simple and Cost-Efficient Two-Photon Microscope for Neuroscience
In this webinar, Max Eisele, Ph.D., gives an introduction to two-photon microscopy and highlights the potential of multiphoton excitation as compared to the standard fluorescence microscopy technology.
Use-case examples are presented that highlight the benefits of two-photon microscopy for deep-tissue and in vivo imaging. After this basic introduction, Max Eisele guides attendees through the setup of a simple and cost-efficient microscope, including a selection of suitable microscopy platforms, as well as an overview on suitable light sources for general and more specific applications. Special emphasis is placed on cost-efficient single-wavelength fiber-lasers and their advantages for dedicated applications — for example, for calcium imaging in neuroscience.
Finally, he provides a hands-on demonstration of integrating a single-wavelength fiber-laser at 920 nm into a two-photon microscope that is optimized to the excitation and detection of green fluorescent proteins, typically used in neuroscience applications. Don’t miss this opportunity: Learn about the benefits of two-photon microscopy, learn about a simple two-photon microscope design, and see how easy and quick it is to set up a dedicated two-photon microscope.