LaVision-Biotec new generation UltraMicroscope II: Light Sheet Microscopy

UltraMicroscope II

In 2009 the first commercial light sheet microscope was launched by LaVision BioTec. Now, the second generation of their light sheet microscope has been released, inspired by user’s feedback. 

The new UltraMicroscope II utilizes six thin light sheets to excite samples while the fluorescence light is detected with a CMOS equipped microscope that is mounted perpendicular to the plane illumination. By moving the sample through the light sheets one generates a 3D image stack at cellular resolution. 

UM 2 chamber

The UltraMicroscope II is the only light sheet microscope which can image samples in aqueous buffers and in organic solvents. The light sheet microscope can image samples ranging in size from few µm up to more than 1 cm. Innovative light sheet forming optics can cover the entire field of view with a homogenous thin light sheet. In case of large samples no stitching is required due to a FOV diagonal from 1.7 mm to 17 mm.

The 10x zoom optic allows fast switching in between smallest to highest magnification without changing objective lenses. Different working distances from 4 mm to 10 mm are available. The UltraMicroscope II is widely used by facilities, institutes and pharmaceutical companies because it is flexible, robust and easy to operate.

Imaging Solutions: organic solvents and aqueous buffer

Sample Size: a few µm up to more than 1 cm

Working Distance: 4 mm, 6 mm, 10 mm

Clearings: Scale, SeeDB, CLARITY, THF & DBE

For further details, please visitLaVision Biotec.

Download pdf brochure here.

Light Sheet Microscopy Article:


The article "A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy" by René Hägerling et al. published in The EMBO Journal (Volume 32, Number 5, 06 March 2013) reveals new insights into the lymphoangiogenesis. We would like to congratulate the researchers on their outstanding results. The cover of The EMBO Journal (2013, 32) displays a series of images acquired with the Ultramicroscope. Thanks to all concerned parties for the interesting data.  

Recent Ultramicroscope publications

DOI: 10.1523/ENEURO.0022-15.2015
Optimization of CLARITY for Clearing Whole-Brain and Other Intact Organs
Jonathan R. Epp, Yosuke Niibori, Hwa-Lin (Liz) Hsiang, Valentina Mercaldo, Karl Deisseroth, Sheena A. Josselyn, Paul W. Frankland

Blood. 2015 Jun 11;125(24):3769-77. doi: 10.1182/blood-2014-09-603803. Epub 2015 Apr 23.
Podoplanin and CLEC-2 drive cerebrovascular patterning and integrity during development.
Lowe KL, Finney BA, Deppermann C, Hägerling R, Gazit SL, Frampton J, Buckley C, Camerer E, Nieswandt B, Kiefer F, Watson SP. 

PLoS One. 2015 Apr 7;10(4):e0122976. doi: 10.1371/journal.pone.0122976. ECollection 2015.
A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research.
Bianchi R, Teijeira A, Proulx ST, Christiansen AJ, Seidel CD, Rülicke T, Mäkinen T, Hägerling R, Halin C, Detmar M. 

eneuro; DOI: 10.1523/ENEURO.0001-15.2015
3D imaging of axons in transparent spinal cords from rodents and non-human primates
Soderblom C, Lee DH, Dawood A, Carballosa M, Santamaria AJ, Benavides FD, Jergova S, Grumbles R, Thomas C, Park K, Guest JD, Lemmon V, Lee JK, Tsoulfas P.  

Cell. 2014 Nov 6;159(4):896-910
iDISCO: A Simple, Rapid Method to Immunolabel Large Tissue Samples for Volume Imaging.
Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-Lavigne M. 

Cell. 2014 Nov 6;159(4):911-24
Whole-body imaging with single-cell resolution by tissue decolorization.
Tainaka K, Kubota SI, Suyama TQ, Susaki EA, Perrin D, Ukai-Tadenuma M, Ukai H, Ueda HR. 

Cell Rep. 2014 Nov 20;9(4):1191-201.
A Simple Method for 3D Analysis of Immunolabeled Axonal Tracts in a Transparent Nervous System.
Belle M, Godefroy D, Dominici C, Heitz-Marchaland C, Zelina P, Hellal F,  Bradke F, Chédotal A 

Mol Cell Biol. 2014 May;34(9):1634-48. doi: 10.1128/MCB.01526-13. Epub 2014 Feb 24.
Fusing VE-cadherin to ?-catenin impairs fetal liver hematopoiesis and lymph but not blood vessel formation.
Dartsch N, Schulte D, Hägerling R, Kiefer F, Vestweber D.

Cell. 2014 Apr 24
Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis
Susaki EA, Tainaka K, Perrin, Kishino F, Tawara T, Watanabe TM, Yokoyama C, Onoe H, Eguchi M, Yamaguchi S, Abe T, Kiyonari H, Shimizu Y, Miyawaki A, Yokota H, Ueda HR.

Cancer Res. 2014 Apr
Apoptosis Imaging for Monitoring DR5 Antibody Accumulation and Pharmacodynamics in Brain Tumors Noninvasively
Weber TG, Osl F, Renner A, Pöschinger T, Galbán S, Rehemtulla A, Scheuer W.

Invest Radiol. 2014 Mar
Dynamic Contrast-Enhanced Micro-Computed Tomography Correlates With 3-Dimensional Fluorescence Ultramicroscopy in Antiangiogenic Therapy of Breast Cancer Xenografts
Pöschinger T, Renner A, Eisa F, Dobosz M, Strobel S, Weber TG, Brauweiler R, Kalender WA, Scheuer W. 

Neoplasia. 2014 Jan
Multispectral fluorescence ultramicroscopy: three-dimensional visualization and automatic quantification of tumor morphology, drug penetration, and antiangiogenic treatment response
Dobosz M, Ntziachristos V, Scheuer W, Strobel S

The EMBO Journal. 2013 May
A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy
René Hägerling, Cathrin Pollmann, Martin Andreas, Christian Schmidt, Harri Nurmi, Ralf H Adams, Kari Alitalo, Volker Andresen, Stefan Schulte-Merker and Friedemann Kiefer

Experimental Neurology. 2013 Mar
Three-dimensional evaluation of retinal ganglion cell axon regeneration and pathfinding in whole mouse tissue after injury
Luo, X., Salgueiro, Y., Beckerman, S. R., Lemmon, V. P., Tsoulfas, P., & Park, K. K. 

Nature protocols. 2012 Oct
Three-dimensional imaging of solvent-cleared organs using 3DISCO
Ali Ertürk, Klaus Becker, Nina Jährling, Christoph P Mauch, Caroline D Hojer, Jackson G Egen, Farida Hellal, Frank Bradke, Morgan Sheng & Hans-Ulrich Dodt

LaVision Biotec Ultramicroscope

LaVision Biotec's Ultramicroscope represents an entirely new class of imaging system, created by combining pioneering ideas from the last century with today's technical concepts. It offers exceptional new opportunities for:

· Whole brain imaging at cellular resolution
· 3D high speed imaging without bleaching
· High throughput phenotype screening

With LaVision BioTec’s Ultramicroscope, you can handle samples even a centimeter in size, yet with sub-cellular resolution. Based on a technique originally developed for particle analysis in 1903*, it has been refined this century for optical sectioning of large biological samples.

LaVision BioTec’s Ultramicroscope utilizes a thin sheet of light to excite biological samples while the fluorescence light is detected with a CCD-equipped microscope perpendicularly to the illuminated area. By moving the sample through the sheet and taking pictures at each depth, one generates a 3D image at cellular resolution in whole organs, using fluorescent proteins, fluorescent dyes or autofluorescence!

To date juvenile mouse brain, complete mouse embryos as well as whole fruit flies have been successfully analyzed. A few articles have yet been published based on sheet illumination of biological samples, so the field is open! Whether you want to chart neuronal networks, characterize phenotypes, study vascularization, or something else as yet untested, this is the emerging technology for you!

The Ultramicroscope can be customized to your application! Please ask for: high speed cameras, incubation chambers for in vivo work and high resolution optics.

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