The Topticamulti-laser engine iChrome CLE for biophotonics provides 405, 488, 561 and 640 nm with 20 mW at the end of the fiber. The cost-effective system is ideal for multi-color microscopy applications due to its compact design and economic operation. Even the 561 nm light is generated by a laser diode instead of a solid-state laser, allowing this system to be directly modulated at high speed (1 MHz) while maintaining complete-off, i.e. zero photons, in the dark state. The iChrome CLE is the latest member of TOPTICA’s iChrome product line which also includes the powerful iChrome MLE (up to 100 mW and four laser lines). All iChrome systems have a unified user interface, unique modulation features and COOLAC, TOPTICA’s proprietary and fully automated beam alignment algorithm guaranteeing consistent power out of the fiber.