Many eyes see more than one – this is the motto behind the brand new MATRIX detectors from Abberior Instruments. Numerous photodiodes integrated in a single detector chip view the sample from different angles and allow you to see things that you couldn’t otherwise. The MATRIX detector drastically improves signal-to-background ratio, resolution, and dynamic range!
- Effective background reduction without artefact-prone mathematical estimation algorithms
- Highly improved optical sectioning
- Enables precise analysis of your image data
Our MATRIX detectors consist of multiple avalanche photo diodes (APDs) with an extraordinarily high quantum efficiency (over 50 % at 500 nm) in a hexagonal arrangment on a single detector chip. Essentially, taking STED images with over 20 instead of just one eye unravels a whole new dimension of data.
Recording STED images with point detectors typically leads to images with high background levels, because the STED-donut throws out low-resolution signal in the focal plane (after all, this is what it’s supposed to do), while it leaves the background largely untouched. Since STED microscopy is a scanning method, a confocal pinhole can be used to improve the signal-to-background ratio, but for many samples, this is not enough.
With MATRIX detection, the background contribution for every pixel is measured and subtracted using physical principles. It’s not at all just another type of estimation procedure such as deconvolution or denoising. Due to its over 20 elements, the MATRIX detectors can truly measure the amount of background and remove it in the best possible way.
This way, you get STED images with drastically reduced background levels. And this in turn hugely improves analysis and post-processing, whether its particle counting, size estimation, intensity measurements, smoothing, or deconvolution. All this works better, because MATRIX images provide 100% physical superresolution without background fluorescence.
Just like our standard APDs, the MATRIX detectors work with our RAINBOW spectral detection, which allows you to freely choose the detection band for every MATRIX. Yes, of course you can have more than one in a system, why?
Images below: Cultured mammalian cells (NUP, Giantin, and Vimentin, labeled with abberior STAR Red, STAR Orange, STAR Green). Out-of-focus contributions of all three types of structures is effectively removed and section is improved. In each pair, the top image is Conventional STED, and below, MATRIX Sted.