The Abberior Instruments STEDYCON now supports motorized stages for stitched fields up to many millimeters.
For a full overview over even millimeter-sized samples, a tiled spiral scan is generated, which allows navigation and imaging within the whole context of your specimen. Subsequently, multiple regions of interest (ROI) can be placed in order to queue the automatic recording of many images with confocal or STED, while you go on and use the imaging time to prepare the next sample. Large ROIs are acquired as tile scan and can be easily stitched using, e.g., SVI Huygens.
Confocal Imaging of large sample regions
Shown is a 2.8x2.5 mm region, acquired using 35 by 29 tiles with the Steadyfocus focus-lock and stitched with SVI Huygens. Sample: mammalian cells immunolabelled with antibodies for the mitochondrial protein TOM20 (Star RED, red), double stranded DNA to visualize mitochondrial DNA (Star ORANGE, green), Phalloidin to label F-actin (Star Green, gray), and DAPI to label nuclei.
Superresolution STED Imaging of large sample regions. The option to combine STED imaging with tiled scanning and stitching facilitates the discovery of rare events such as cell division. Sample: mammalian cells immunolabelled with antibodies for the mitochondrial protein TOM20 (Star RED, red) and double stranded DNA to visualize mitochondrial DNA (Star ORANGE, green). The tiles were acquired with the Steadyfocus focus-lock and stitched using SVI Huygens.
Confocal acquisition of Convallaria rhizome (cross section) with an 20x oil objective; 3 channels, maximum intensity projection. The images consist of 14 z-planes in 9 by 9 tile pattern comprising a total area of 3.2x3.2 mm, stitched using SVI Huygens. Shown is a maximum intensity projection.
Kernel of zea mays corn, longitudinal section stained with toluidine blue. Imaged with 640 nm, 561 nm and 488 nm excitation lasers and 100x magnification. The image consists of 441 tiles and was stitched using SVI Huygens.