The RWD Life Science RFLSI-ZW laser speckle imaging system is the successor to the RFLSI-III, and offers an even better tool for microcirculation research based on laser speckle contrast imaging technology (LSCI). With its advanced optical design and improved image processing algorithm, RFLSI-ZW shows greater performance in imaging field size, image quality, full-field frame rate and optical resolution, and provides a powerful and efficient means for human and animal tissue blood flow measurement.

A demonstration system is currently available from Lastek for obligation-free trial.


The LSCI technology advantages are its non-contact, high frame-rate, high spatial resolution. They can be used to observe and record blood perfusion of any exposed tissues or organs for microcirculation study or pre-clinical researches like ischemic stroke, lower limbs, mesentery, etc. Multi-Output includes blood perfusion images and videos (500+ million pixels), quantified data for perfusion unit and vessel diameter.

Since 2019, our imaging system has been adopted by more than 100 colleges, universities, and research institutes worldwide such as Stanford University School of Medicine, university of manchester, UC Davis, Duke university. What’s more, it has contributed to publishing more than 100 reputed research papers in magazines like Nature communications, Blood, Diabetes, and Theranostic.

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  • Cerebral blood perfusion monitoring
  • MCAO model assessment
  • Cortical spreading depression observation
  • Hind-limb ischemia research
  • Skin burn/skin flap transplantation
  • Organ microcirculation observation
  • Skin allergies
  • Septic Shock
  • Chicken Chorioallantoic Membrane Assay
  • Diabetic Foot

Highlights Of RFLSI-ZW

  • Image any exposed tissue (skin or surgically exposed tissues) and species.
  • Non-contact, non-contrast agent depending measurement.
  • The built-in CMOS global shutter camera can achieve faster data acquisition and processing speed.
  • Best optical resolution of 3.9 μm/pixel, providing more detailed tissue structures.
  • Max frame rate (full field) up to 100 fps, acquiring real-time changes in larger areas.
  • Motorised 10x optical zoom and auto focus. Image size ranges from 0.57×0.75 to 22.5×30 cm2 in all-in-one imager, covering multiple research applications.
  • Fast auto and fine manual focus, improving focus efficiency and accuracy on various tissues.
  • Optimal lens assembly, filtering the ambient and reflecting light.
  • Class 1 of measurement and indicating lasers, safe to use without eye protection System
  • Laser stability hardware for the ultimate in reliable and consistent measurement over minutes, hours and days.
  • Calibration with calibration box. Self-calibration is possible at any time to keep the equipment in optimal working condition.
  • Trigger In/Out BNC connections for communication with external devices.
  • Unlimited installation of analysis software in PC.


RWD laser speckle has contributed to publishing more than 100 reputed research papers in journals like Nature Communications, Blood, Circulation Research, Brain, Diabetes, and Theranostic. The paper list we collect contains about 18 research topics such as Cerebral Ischemia & Ischemic Stroke, Traumatic Brain Injury (TBI), Angiogenesis, Diabetes, Alzheimer’s Disease, Wound Healing, Limb Ischemia, etc.

Download publications list here

Publication highlights:

Journal: Nature Neuroscience

Use of the Laser Speckle Imaging System (LSCI) to record blood flow changes in the barrel cortex of adult mice receiving tamoxifen for detecting the changes of regional CBF mediated by Glu-NsMJ sensory input-evoked. More here

Journal: Circulation Research

Use of the Laser Speckle Imaging System to measure blood flow in cerebral arteries via a cranial window in anesthetized mice, to corroborate an in vivo physiological role for pS1928 upon HG. More here.

Journal: Brain

Use of laser speckle imaging to corroborate haemodynamic changes within the targeted region on the ipsilateral side of the brain before and after stroke in the photothrombotic stroke model. More here