Becker & Hickl PML-16 GaAsP devices are 16-channel TCSPC detectors with highly efficient GaAsP cathodes. Signal recording is based on bh’s multi-dimensional TCSPC process. For every photon, the detector delivers a timing pulse and the number of the channel that detected the photon. From this information, the TCSPC module builds up a photon distribution over the times of the photons in the signal period and the channel number. The results is a set of individual optical signal waveforms for the 16 channels of the detector.

The PML-SPEC-GaAsP and MW-FLIM-GaAsP devices are combinations of the PML-16 GaAsP detectors with a polychromator. The polychromator splits the optical signal into its spectral components. These are detected by the 16 channels of the detector. The results is a set of optical waveforms for 16 wavelength channels. The PML-SPEC has a free-beam or an optical-fibre input, the MW-FLIM has a fibre-bundle input for connecting to non-descanned ports of multiphoton microscopes. All detectors connect directly to the bh TCSPC modules or the bh Simple-Tau systems, see below.

The PML-16 GaAsP, PML-SPEC GaAsP, and MW-FLIM GaAsP detectors are about 6 times more sensitive than their PML-16C predecessors with conventional cathodes. A comparison is shown in the figures below.

Ftxy convall pml16c 02 comp80Ftxy convall gaasp02 comp80

Multi-wavelength fluorescence decay recording. Left: PML-16C (multialkali). Right: PML-16 GaAsP (gallium arsenide phosphide). Same acquisition time and intensity scale.

flim pml spec 01 max1000 comp80

 

flim pml spec GaAsP max1000 rr comp160

Multi-wavelength FLIM, 8 of 16 channels shown. Top: MW FLIM (multialkali). Bottom: MW FLIM GaAsP. Same acquisition time, same intensity scale.