STED

Abberior 2-color STED 595 QUAD Scanning

 

Two-color superresolution STED microscope with a pulsed STED laser @ 595 nm

  • Multicolor confocal scanning system with variable pinhole size
  • Detector gating with up to 4 gates
  • Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
  • Zero-compromize on performance - get the best resolution performance possible in STED microscopy
  • QUAD beam scanner technology
  • We offer unrivaled flexibility in customizing the microscope to your applications

We install Your turn-key Abberior 2-color STED 595 QUAD scanning nanoscope in your lab including software/handling training.

Abberior specifications:

  • Pulsed STED laser @ 595nm featuring two super-resolution STED channels
  • Maximum resolution 25 nm; typical resolution < 40 nm with organic dyes
  • Kit with all optical and mechanical components for assembly of a 2-color STED 595nm system onto an Olympus IX83 microscope (or other stand on request)
  • IX83 Olympus microscope with a 4-color LED illumination source and a monochrome widefield camera
  • Ultracompact Abberior QUAD scanner with 4 galvo mirrors (with 4 degrees of freedom position and angle independently addressable) and a speed up to 2 kHz line frequency
  • Abberior Patchpanel: electronic connectors (AO, AI, DIOs) for attaching custom external devices (e.g. laser sources etc.)
  • Two pulsed excitation lasers
  • Suitable for superresolution imaging with fluorescent proteins, e.g. GFP, YFP as well as imaging with organic dyes, e.g. Abberior STAR 440SX or STAR 488
  • Up to 8 time gating channels with <80 ps time resolution
  • Up to 4 pulse-timing channels with <80 ps time resolution
  • Multi-color acquisition method via interleave mode
  •           (i) pulse interleaved and/or
  •           (ii) pixel interleaved and/or
  •           (iii) line-interleaved
  • Data acquisition system and software for image acquisition for different imaging modes (including 2-color STED mode)
  • Software for data analysis (e.g. deconvolution algorithms etc.)

Downloads:

Comprehensive Brochure

Abberior Multi-color STED @ 595nm

Abberior Quad Scanner

Controlling Platform for Abberior Microscopes

Dimensions

Imspector Software platform

References:

Clausen, M.P., Galiani, S., de la Serna, J.P., Fritzsche, M., Chojnacki, J., Gehmlich, K., Lagerholm, B.C., Eggeling, C.: "Pathways to optical STED microscopy", DOI: 10.2478/nbi-2013-0001, NanoBioImaging, 1-12 (2013)

Rankin, B.R., Moneron,G., Wurm C.A., Nelson, J.C., Walter, A., Schwarzer, D., Schroeder, J., Colo´n-Ramos, D.A. and Hell, S.W.: "Nanoscopy in a Living Multicellular Organism Expressing GFP", Biophysical J. Volume 100, L63–L65 (2011)

Tønnesen, J., Nadrigny, F., Willig, K.I., Wedlich-Söldner, R. and Nägerl, V.: "Two-Color STED Microscopy of Living Synapses Using A Single Laser-Beam Pair", Biophys J., 101(10): 2545–2552 (2011)

Hein, B., K. I. Willig, S. W. Hell: "Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell" PNAS 105, 14271-14276 (2008)

Willig, K.I., Rizzoli, S.O., Westphal, V., Jahn, R., and Hell, S.W.: "STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis", Nature, Vol. 440|13 (2006)

Donnert, G., J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Lührmann, R. Jahn, C. Eggeling, S. W. Hell: "Macromolecular-scale resolution in biological fluorescence microscopy" PNAS 103, 11440-11445 (2006)

 

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