New from Abberior Instruments: RESCue STED!

Fig7 tubu red

Abberior Instruments RESCue STED is a STED imaging mode dedicated to live-cell imaging with saving up to 96% of the light dose without compromising the resolution.  

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Abberior Instruments introduce DyMIN STED: Live-cell STED with Dynamic Minimum intelligent light dose management

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Abberior Instruments announce DyNIM STED: Dynamic Minimum intelligent light dose management for live cell imaging.

Key Benefits of DyMIN STED:

  • The only live-cell superresolution microscope with resolutions down to 25 nm* (*only depending on the sample) - DyMIN STED dramatically reduces the light irradiation on your sample (up to two orders of magnitude) and features the lowest light dose of all superresolution methods.
  • Resolution truly down to 25 nm - As demonstrated by separating two fluorescent point-structures being 30 nm apart.
  • Volume / time-lapse imaging with easy3D STED resolution - DyMIN STED substancially reduces photobleaching and enables long term measurements over volumes or over dozens and dozens of frames.

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Becker & Hickl and Abberior Combination Records STED FLIM at Megapixel Resolution

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The combination of the Abberior Instruments STED system with the Becker & Hickl Simple-Tau 150/154 TCSPC FLIM system records FLIM data at a spatial resolution of better than 40 nm. The image format can be as large as 2048 x 2048 pixels, with 256 time channels per pixel. An image area of 40 x 40 micrometers can thus be covered with 20 nm pixel size, fully satisfying the Nyquist criterion. The system benefits from Windows 64 bit technology used both in the Abberior and in the bh data acquisition software, from the combined processing power of the two system computers, and the high data throughput of up to four parallel TCSPC FLIM channels. The system achieves peak count rates in excess of 5 MHz per FLIM channel, resulting in unprecedented signal-to-noise ratio and short acquisition time.

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Abberior Instruments Protected STED technique enhances resolution and contrast in live-cell STED microscopy

neuron

3D-protected STED imaging of neuron in living brain tissue. 3D-rendering of a 3D-image stack of a living neuron expressing the AC-rsEGFP2 actin label in a hippocampal brain slice. Protected STED was applied with resolution enhancement in the xy and z directions. The image shows a stretch of dendrite with dendritic spines. The stack corresponds to 56 z slices spaced by 80 nm. Scale bar: 1 µm.

Abberior Instruments Protected STED technique has been highlighted in a new January 2016 paper in Nature Photonics. Full paper available for download here.

Coordinate-targeted Fluorescence nanoscopy with multiple off states

Johann G. Danzl*†, Sven C. Sidenstein†, Carola Gregor, Nicolai T. Urban, Peter Ilgen, Stefan Jakobs and Stefan W. Hell*

Nature Photonics: Published Online 18 January 2016 DOI: 10.1038/NPHOTON 2015.266

Abstract: By exploiting a second off state of a reversibly switchable fluorophore, a general approach that can reduce photobleaching and enhance resolution of coordinate-targeted fluorescence nanoscopy has been demonstrated.

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Correlative STED + AFM: Abberior Instruments integrate STED with JPK NanoWizard AFM

AFM STED 690

Abberior Instruments and JPK Instruments have successfully integrated the Abberior Instruments STED microscope and the JPK NanoWizard® 4 AFM, to achieve correlative STED + AFM.

  • Correlative STED + AFM measurements
  • Several STED and confocal imaging modes (2D & 3D STED, single & multi color, time laps, 3D stacks, FLIM, RESCue)
  • Several AFM imaging modes (including AFM manipulation modes)
  • Simultaneous STED and AFM imaging possible
  • Very high accuracy (~15 nm) of the DirectOverlayTM (function of the JPK software) of the STED and AFM image
  • AFM manipulation with subdiffraction positioning accuracy.

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Abberior Instruments easy3D STED: Explore the 3rd dimension

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  • Spatial Light Modulator (SLM) technology
  • Resolution tunable between xy and z
  • Single STED beam
  • Enables the use of different objective lenses
  • Resolution typically 80 × 80 × 90 nm
  • Engineered for maximum stability

Abberior Instruments is proud to announce its new easy3D STED module which uses a completely novel approach to 3D STED microscopy. At its core, a programmable spatial light modulator (SLM) is used to create the phase patterns required for 2D and 3D STED microscopy. At the same time, it can also be used to correct for optical aberrations.

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Abberior STEDYCON: Imaging beyond barriers

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Abberior Instruments is known for extraordinary customization ability of its flexible STED microscope platform. We now take things in a different direction. We have developed a completely new instrument, the ultimate compact STED plus confocal microscope, called STEDYCON.

STEDYCON is a completely new class of nanoscope. It converts your conventional epifluorescence microscope into a powerful multicolor confocal and STED system! At the same time, it is incredibly compact and can be used by anyone!

  • Convert your existing microscope - Instantly upgrade any epifluorescence microscope body to multicolor confocal and STED capabilities.
  • Get top-notch STED performance - With STEDYCON your microscope will image at 30nm resolution.
  • Save Space - The incredibly compact STEDYCON comes at the size of a camera.
  • Plug & Play - The STEDYCON is assembled within minutes. It simply connects to the C-mount camera port of your microscope with no need for optical alignment.
  • Service and maintenance free - The laser beams of the STEDYCON are aligned by design as provided by its patent-protected 'easySTED' optical arrangement.
  • Innovative web-based user interface - The STEDYCON can be controlled via a browser on any PC, MAC or even a tablet!

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Abberior Instruments 1C RESOLFT QUAD Scanning

Sample: keratin19 (rsCherryRev1.4, red) and vimentin (Dronpa-M159T, green)
Resolution RESOLFT < 80 nm  

One-color superresolution green RESOLFT point scanning system for switchable GFPs

  • Multicolor confocal system with variable pinhole size
  • Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
  • Zero-compromize on performance - get the best resolution performance possible in RESOLFT microscopy
  • QUAD beam scanner technology
  • We also offer unrivaled flexibility in customizing the microscope to your applications.

We install your turn-key RESOLFT scanning nanoscope in your lab including software/handling training.

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Correlative STED + AFM: Abberior Instruments integrate STED with JPK NanoWizard AFM

AFM STED 690

Abberior Instruments and JPK Instruments have successfully integrated the Abberior Instruments STED microscope and the JPK NanoWizard® 4 AFM, to achieve correlative STED + AFM.

  • Correlative STED + AFM measurements
  • Several STED and confocal imaging modes (2D & 3D STED, single & multi color, time laps, 3D stacks, FLIM, RESCue)
  • Several AFM imaging modes (including AFM manipulation modes)
  • Simultaneous STED and AFM imaging possible
  • Very high accuracy (~15 nm) of the DirectOverlayTM (function of the JPK software) of the STED and AFM image
  • AFM manipulation with subdiffraction positioning accuracy.

Read more ...

Abberior fluorophores for superresolution microscopy

20150814 Abberior OverviewMarkers

 

Above: Full listing of available fluorophores from Abberior. Please click on image to open full-size in new window.

Abberior GmbH offers fluorescent dyes and labels that are exceptionally well suited for superresolution microscopy such as STED, GSD, PALM, STORM, GSDIM, SIM, and RESOLFT. They also give outstanding results in confocal and epifluorescence microscopy as well as in single-molecule applications. Our mission is to become the leading supplier for fluorescent labels in superresolution microscopy/nanoscopy.

In recent years, fluorescence microscopy has undergone a revolution: the resolution barrier given by the diffraction limit was broken by new microscopy concepts such as STED, GSD, RESOLFT, PALM, STORM, GSDIM. All of these concepts are based on the proper design and utilization of the photophysical /photochemical properties of the dye. Hence, the dye plays a key role for obtaining the ultrasharp pictures with these methods.

Based on more than a decade of research experience in superresolution microscopy / fluorescence nanoscopy and a passion for developing new, functionalized dyes for this new era of microscopy, the Abberior team is best positioned to offer fluorophore labels for the most demanding, cutting-edge nanoscopy/superresolution applications. Abberior uses patent-protected technology and will continue to invest into the development of new products.

Visit Abberior on-line shop here or click on image below.

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