Abberior Instruments Protected STED technique enhances resolution and contrast in live-cell STED microscopy

neuron

3D-protected STED imaging of neuron in living brain tissue. 3D-rendering of a 3D-image stack of a living neuron expressing the AC-rsEGFP2 actin label in a hippocampal brain slice. Protected STED was applied with resolution enhancement in the xy and z directions. The image shows a stretch of dendrite with dendritic spines. The stack corresponds to 56 z slices spaced by 80 nm. Scale bar: 1 µm.

Abberior Instruments Protected STED technique has been highlighted in a new January 2016 paper in Nature Photonics. Full paper available for download here.

Coordinate-targeted Fluorescence nanoscopy with multiple off states

Johann G. Danzl*†, Sven C. Sidenstein†, Carola Gregor, Nicolai T. Urban, Peter Ilgen, Stefan Jakobs and Stefan W. Hell*

Nature Photonics: Published Online 18 January 2016 DOI: 10.1038/NPHOTON 2015.266

Abstract: By exploiting a second off state of a reversibly switchable fluorophore, a general approach that can reduce photobleaching and enhance resolution of coordinate-targeted fluorescence nanoscopy has been demonstrated.

Read more ...

Becker & Hickl and Abberior Combination Records STED FLIM at Megapixel Resolution

microtubules sted intervalfit rr comp80

The combination of the Abberior Instruments STED system with the Becker & Hickl Simple-Tau 150/154 TCSPC FLIM system records FLIM data at a spatial resolution of better than 40 nm. The image format can be as large as 2048 x 2048 pixels, with 256 time channels per pixel. An image area of 40 x 40 micrometers can thus be covered with 20 nm pixel size, fully satisfying the Nyquist criterion. The system benefits from Windows 64 bit technology used both in the Abberior and in the bh data acquisition software, from the combined processing power of the two system computers, and the high data throughput of up to four parallel TCSPC FLIM channels. The system achieves peak count rates in excess of 5 MHz per FLIM channel, resulting in unprecedented signal-to-noise ratio and short acquisition time.

Read more ...

Abberior Instruments introduce FLIM upgrade for STED microscopes

 Headline FIG small 650

Abberior Instruments STED microscope now available with full Fluorescence Lifetime IMaging (FLIM) functionality!

The new Abberior Instruments comprehensive FLIM package upgrade offers the following key features:

  • STED FLIM imaging in combination with our pulsed STED lasers
  • Online display of STED FLIM images during the measurement
  • Online lifetime calculation and/or dye separation based on lifetime data
  • Simultaneous FLIM acquisition in up to 4 spectral channels (based on Becker & Hickl SPCM hardware)
  • Full software integration into Imspector including evaluation functionality (also live evaluation of data)

Continue below to see a selection of images generated using the Abberior STED FLIM technique.

Read more ...

Correlative STED + AFM: Abberior Instruments integrate STED with JPK NanoWizard AFM

AFM STED 690

Abberior Instruments and JPK Instruments have successfully integrated the Abberior Instruments STED microscope and the JPK NanoWizard® 4 AFM, to achieve correlative STED + AFM.

  • Correlative STED + AFM measurements
  • Several STED and confocal imaging modes (2D & 3D STED, single & multi color, time laps, 3D stacks, FLIM, RESCue)
  • Several AFM imaging modes (including AFM manipulation modes)
  • Simultaneous STED and AFM imaging possible
  • Very high accuracy (~15 nm) of the DirectOverlayTM (function of the JPK software) of the STED and AFM image
  • AFM manipulation with subdiffraction positioning accuracy.

Read more ...

Introducing: Abberior Instruments

Lastek is delighted to announce that it has been appointed Asia-Pacific distributor for Abberior Instruments

Co-founded by 2014 Nobel Prize in Chemistry winner, Professor Stefan W. Hell, Abberior aims to become the leading supplier of cutting-edge super-resolution technology with the best possible resolution performance. In combination with their sister company Abberior, they offer an unrivaled combination of superresolution dyes and nanoscopy hardware.

Why work with Abberior Instruments and Lastek?

  • Connect with the inventors of STED and RESOLFT
  • Get the best possible resolution performance
  • Development cycles less than one year: stay cutting-edge with your upgradable system from Abberior Instruments
  • Why buy an outdated confocal/widefield microscope when you can get the latest superresolution technology for the same price?

Read more ...

Abberior Instruments introduce FLIM upgrade for STED microscopes

 Headline FIG small 650

Abberior Instruments STED microscope now available with full Fluorescence Lifetime IMaging (FLIM) functionality!

The new Abberior Instruments comprehensive FLIM package upgrade offers the following key features:

  • STED FLIM imaging in combination with our pulsed STED lasers
  • Online display of STED FLIM images during the measurement
  • Online lifetime calculation and/or dye separation based on lifetime data
  • Simultaneous FLIM acquisition in up to 4 spectral channels (based on Becker & Hickl SPCM hardware)
  • Full software integration into Imspector including evaluation functionality (also live evaluation of data)

Continue below to see a selection of images generated using the Abberior STED FLIM technique.

Read more ...

Becker & Hickl and Abberior Combination Records STED FLIM at Megapixel Resolution

microtubules sted intervalfit rr comp80

The combination of the Abberior Instruments STED system with the Becker & Hickl Simple-Tau 150/154 TCSPC FLIM system records FLIM data at a spatial resolution of better than 40 nm. The image format can be as large as 2048 x 2048 pixels, with 256 time channels per pixel. An image area of 40 x 40 micrometers can thus be covered with 20 nm pixel size, fully satisfying the Nyquist criterion. The system benefits from Windows 64 bit technology used both in the Abberior and in the bh data acquisition software, from the combined processing power of the two system computers, and the high data throughput of up to four parallel TCSPC FLIM channels. The system achieves peak count rates in excess of 5 MHz per FLIM channel, resulting in unprecedented signal-to-noise ratio and short acquisition time.

Read more ...

Abberior Instruments 2-color STED 775 QUAD Scanning

 

Abberior Instruments presents two-color superresolution STED microscope with a pulsed STED laser @ 775 nm

  • Multicolor confocal scanning system with variable pinhole size
  • Detector gating with up to 4 gates
  • Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
  • Zero-compromize on performance - get the best resolution performance possible in STED microscopy
  • QUAD beam scanner technology
  • We offer unrivaled flexibility in customizing the microscope to your applications

    Read more ...

Abberior 2-color STED 595 QUAD Scanning

 

Two-color superresolution STED microscope with a pulsed STED laser @ 595 nm

  • Multicolor confocal scanning system with variable pinhole size
  • Detector gating with up to 4 gates
  • Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
  • Zero-compromize on performance - get the best resolution performance possible in STED microscopy
  • QUAD beam scanner technology
  • We offer unrivaled flexibility in customizing the microscope to your applications

We install Your turn-key Abberior 2-color STED 595 QUAD scanning nanoscope in your lab including software/handling training.

Read more ...

Abberior Instruments: New Live-Cell STED Package

live cell package.1280x0

Recording living cells is a challenge all by itself. Additionally, when doing live-cell super-resolution imaging on living cells, there is a multitude of things to take care of at the same time.  

To enable you to focus on getting the best images, Abberior Instruments offers a unique live-cell package with its STED and RESOLFT microscopes including a continuous STED autofocus, RESCue-STED and more:

Read more ...

You are here: Home Microscopy Superresolution Microscopy