FLIM recorded by bidirectional scanning. Above: FLIM of Convallaria sample, 512x512 pixels.
Starting from Version 9.73 SPCM Software, the Becker & Hickl TCSPC / FLIM systems are able to record FLIM with bidirectional scanning. The data are recorded in the ‘FIFO Imaging’ mode. The data acquisition is synchronised with the scanning by frame clock, line clock, and pixel clock pulses from the scanner. Each first line clock pulse indicates the beginning of a forward scan, each second one the beginning of a backward scan. The recording procedure automatically reverses the data from the backward scan and compensates for the line shift caused by the dynamic behaviour of the scanner.