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 npc lamina teaser 1.1280x448

Abberior Instruments announce DyNIM STED: Dynamic Minimum intelligent light dose management for live cell imaging.

Key Benefits of DyMIN STED:

  • The only live-cell superresolution microscope with resolutions down to 25 nm* (*only depending on the sample) - DyMIN STED dramatically reduces the light irradiation on your sample (up to two orders of magnitude) and features the lowest light dose of all superresolution methods.
  • Resolution truly down to 25 nm - As demonstrated by separating two fluorescent point-structures being 30 nm apart.
  • Volume / time-lapse imaging with easy3D STED resolution - DyMIN STED substancially reduces photobleaching and enables long term measurements over volumes or over dozens and dozens of frames.

 fig 2 dreicke neu.900x0

fig 3 bleichverlauf.900x0

 

Inspired by our unique RESCue concept DyMIN STED further reduces sample areas where maximum STED power is applied. This reduces the overall bleaching and enables to increase the reachable optical resolution with STED microscopy.

A second conceptual ingredient of DyMIN STED is MINFIELD STED, a co-development between Stefan Hell and coworkers and Abberior Instruments.

DyMIN STED is a multistep probing & imaging concept :

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dymin concept

DyMIN STED complements an existing list of intelligent STED illumination schemes:

grafik kreis vd 01.1280x0

Imaging Results

fig 5 dymin vergleich 1.1280x0

DyMIN STED opens up optical resolutions significantly below 30 nm on the full field of view. DyMIN STED imaging of DNA origami structures at high signal-to-noise ratio clearly separates two fluorescent spots of 30 nm distance (GATTAquant DNA origami sample).

fig 6 dymin conf sted.1280x0

DyMIN STED very clearly resolves spectrin rings with superior signal-to-noise ratio. Primary hippocampal neurons (22 days in vitro) show the characteristic ~192 nm betaII spectrin periodicity along distal axons. Dye: Abberior STAR 635P, Excitation: 635 nm, STED: 775 nm. Sample with courtesy from Elisa D’Este (MPIbpc).

 fig 7 elisarings.900x0

Easy3D DyMIN STED significantly reduces bleaching and thus enables the acquisition of a volume stack with an isotropic resolution of ~85 nm. Primary hippocampal neurons (22 days in vitro), betaII spectrin. Dye: Abberior STAR 635P, Excitation: 635 nm, STED: 775 nm. Sample with courtesy from Elisa D’Este (MPIbpc). Scale bars 200 nm.

 fig 8 npc lamina 1.1280x0

Easy3D DyMIN STED clearly resolves two nuclear pore complex subunits (NPC, green) on the inner and outer side of the nuclear membrane (red). Targets: nup153 (green), lamin (red). Dyes: Alexa 594, Abberior STAR 635P, Excitation: 561nm, 635 nm, STED: 775 nm.

fig 9 vimentin dymin 1.1280x0

After acquisition with DyMIN STED no bleaching is observed. Vimentin labeled with Oregon Green 488; Excitation: 488 nm, STED: 595 nm.

 fig 10 npc vergleich 1.1280x0

After acquisition with DyMIN STED significantly lower bleaching is observed. Nuclear pore complex protein (nup153) labeled with Oregon Green 488; Excitation: 488 nm, STED: 595 nm.

fig 1 npcs

 

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