Recording living cells is a challenge all by itself. Additionally, when doing live-cell super-resolution imaging on living cells, there is a multitude of things to take care of at the same time.
To enable you to focus on getting the best images, Abberior Instruments offers a unique live-cell package with its STED and RESOLFT microscopes including a continuous STED autofocus, RESCue-STED and more:
- Pulsed STED at 595 nm / 775 nm, and
- RESCue STED: They combine to reduce the light dose in your sample to the absolute minimum.
- Water immersion objective lenses: Use the optimal immersion for watery living samples.
- Overnight Remote Control: For automating those tedious late-night experiments.
- Continuous STED autofocus: Stay sharp and in focus over hours.
- Samples may be heated up to 37° using incubation chambers.
All components are fully integrated into our software Imspector.
The full package is in its entirety only available from Abberior Instruments.
Imaging of a Region-of-Interest (ROI) Queue
Below is an example where the Abberior Instruments Live-Cell Package has been used to great effect to consecutively record five pre-defined ROIs. A formidable 100 successive frames where recorded for each region in 50-second intervals before moving on to the next ROI. Thus, each ROI was under the microscope for approx. 1.5 hours and the total acquisition time was about 7.5 hours. During this time, our easy3D-STED microscope ran completely autonomous.
This would not have been possible without all the contents of the Live-Cell Package: Pulsed lasers and RESCue reduced bleaching, the autofocus worked to keep the focus stable, we used water objective lenses and heated the sample to 37°C, and Overnight Control assumed responsibility for automatization.
Five regions of interest were selected in a living sample stained with SiR-Tubulin.
These are the results condensed into five videos from the five ROIs. Crisp STED images at physiological conditions over a time course of more than seven hours. Click on the following links to view the videos online.